Composite

Part:BBa_K4167666:Design

Designed by: Lin Wang   Group: iGEM22_ICJFLS   (2022-10-02)


Toehold switch-LacZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pET-28a-toehold switch-LacZ plasmid was designed to comprise some antisense sequence of miRNA 34a-5p, constant sequence, and part sequence of miRNA 34a-5p, which form a toehold switch, as well as the gene of LacZ. At the presence of miRNA 34a-5p, it binds to its antisense sequence, opening the toehold switch to trigger the expression of β-galactosidase, which can be detected by its substrate X-gal.


Source

miRNA 34a-5p comes from human, LacZ comes from Bacteria.

References