Composite
Part:BBa_K4167666:Design
Designed by: Lin Wang Group: iGEM22_ICJFLS (2022-10-02)
Toehold switch-LacZ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
pET-28a-toehold switch-LacZ plasmid was designed to comprise some antisense sequence of miRNA 34a-5p, constant sequence, and part sequence of miRNA 34a-5p, which form a toehold switch, as well as the gene of LacZ. At the presence of miRNA 34a-5p, it binds to its antisense sequence, opening the toehold switch to trigger the expression of β-galactosidase, which can be detected by its substrate X-gal.
Source
miRNA 34a-5p comes from human, LacZ comes from Bacteria.